Catalog No: OKGD00006
Size:96 Wells
Price: $439.00
SKU
OKGD00006
Availability: Domestic: within 1-2 days delivery | International: 1-2 days
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C3 ELISA Kit (Human) (OKGD00006)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~2.5 hours
ELISA Kit PrincipleAviva Systems Biology C3 ELISA Kit (human) (OKGD00006) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C3 has been pre-coated onto a 96-well plate. Standards or test samples are added to the wells, incubated and washed. A horseradish peroxidase (HRP) conjugated detector antibody specific for C3 is added, incubated and followed by washing. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample C3 captured in well.
ELISA Kit Range3.9 - 500 ng/mL
ELISA Kit Component
ComponentQuantity
Anti-C3 Microplate96 Wells (12 x 8 Well strips)
C3 Lyophilized Standard2 vials
100X C3 HRP-Detection Anitbody1 x 125 uL
Assay Diluent1 x 30 mL
20X Wash Buffer1 x 30 mL
TMB Substrate1 x 12 mL
Stop Solution1 x 12 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Other Biological Fluids
Sensitivity< 3.0 ng/mL
Specificity
Species Reactivity
Species Cross Reactivity (%)
Chicken 0%
Dog 0%
Mouse 5%
Pig 5%
Rat 5%
Cross-Reactivity
Antigen Cross Reactivity (%)
AAT 11%
APOA1 0%
A2M 0%
Fibrinogen 0%
Haptoglobin 0%
HDL 0%
H. HSA 0%
IgA 0%
IgG 0%
IgM 0%
LDL 0%
Orosomucoid 0%
Transferrin 0%
Gene SymbolC3
Gene Full NameComplement C3
Alias SymbolsASP, C3a, C3b, AHUS5, ARMD9, CPAMD1, HEL-S-62p
NCBI Gene Id718
Protein NameComplement C3
Description of TargetComplement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form the mature protein, which is then further processed to generate numerous peptide products. The C3a peptide, also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. Mutations in this gene are associated with atypical hemolytic uremic syndrome and age-related macular degeneration in human patients.
Uniprot IDP01024
Protein Accession #NP_065390
Nucleotide Accession #NM_000014.5
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