Catalog No: OKGD00006
Size:96 Wells
Price: $439.00
SKU
OKGD00006
Availability: Domestic: within 1-2 days delivery | International: 1-2 days
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Application | ELISA | ||||||||||||||||||||||||||||||||||||||||||||
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ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Duration | ~2.5 hours | ||||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology C3 ELISA Kit (human) (OKGD00006) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C3 has been pre-coated onto a 96-well plate. Standards or test samples are added to the wells, incubated and washed. A horseradish peroxidase (HRP) conjugated detector antibody specific for C3 is added, incubated and followed by washing. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample C3 captured in well. | ||||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Range | 3.9 - 500 ng/mL | ||||||||||||||||||||||||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||||||||||||||||||||||||
Sample Type | Serum, Plasma, Other Biological Fluids | ||||||||||||||||||||||||||||||||||||||||||||
Sensitivity | < 3.0 ng/mL | ||||||||||||||||||||||||||||||||||||||||||||
Specificity |
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Gene Symbol | C3 |
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Gene Full Name | Complement C3 |
Alias Symbols | ASP, C3a, C3b, AHUS5, ARMD9, CPAMD1, HEL-S-62p |
NCBI Gene Id | 718 |
Protein Name | Complement C3 |
Description of Target | Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form the mature protein, which is then further processed to generate numerous peptide products. The C3a peptide, also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. Mutations in this gene are associated with atypical hemolytic uremic syndrome and age-related macular degeneration in human patients. |
Uniprot ID | P01024 |
Protein Accession # | NP_065390 |
Nucleotide Accession # | NM_000014.5 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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