Catalog No: OKIA00211
Size:1 x 96 well plate
Price: $400.00
SKU
OKIA00211
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
Product Format12 of 8-well strips
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration60 Minutes
ELISA Kit PrincipleThe Aviva Systems Biology C3 ELISA Kit (Human) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Complement C3  in Plasma or Serum. This assay employs an antibody specific for Human C3 coated on a 96-well plate. Standards and samples are pipetted into the wells and Complement C3  present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-C3 antibodies conjugated with horseradish peroxidase (HRP) are added. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of C3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The quantity of C3 in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.
ELISA Kit Range12.50 - 400 ng/mL
ELISA Kit Reproducibility< 10%
ELISA Kit Component
ComponentAmount
Anti-Human C3 Microplate96 Wells (12 x 8 Well Strips)
Human C3 Calibrator1
100X C3-HRP Conjugate150 uL
5X Assay Diluent50 mL
20X Wash Solution50 mL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Additional InformationIntended Use: The C3 test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring C3/Complement C3 in Human biological samples.
::Sample Volume: 5 uL
Reconstitution and StorageStore at 4 degrees
Sensitivity2.3 ng/mL
Alias SymbolsC3;Complement C3; PZP-like alpha-2-macroglobulin domain-containing protein 1;
Protein NameComplement C3 
Description of TargetC3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes
Uniprot IDP01024
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