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Catalog No: OKEI00882
Size:96 Wells
Price: $625.00
SKU
OKEI00882
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology C1s ELISA Kit (Rat) (OKEI00882) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C1s has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C1s is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample C1s
ELISA Kit Range0.781 - 50 ng/mL
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level C1s. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level C1s using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
LACTB2 Microplate96 Wells (12 x 8 Well strips)
LACTB2 Lyophilized Standard2
100X LACTB2 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 10 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Biological Fluids
Sensitivity0.469 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolC1S
Alias Symbolsr-gsp
NCBI Gene Id192262
Protein NameComplement C1s subcomponent
Description of TargetC1s B chain is a serine protease that combines with C1q and C1r to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4.
Uniprot IDQ6P6T1
Protein Accession #NP_620255
Nucleotide Accession #NM_138900
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