C1S Chemi-Luminescent ELISA Kit (Human) (OKCD03344)

Catalog No: OKCD03344

Size:96WELLS

Human C1S ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	90-98%	89-103%	86-102%	88-101%
  EDTA Plasma (n=5)	96-104%	93-105%	82-95%	79-102%
  Heparin Plasma (n=5)	80-88%	78-105%	94-103%	82-90%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Complement Component 1, S Subcomponent (C1s). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Complement Component 1, S Subcomponent (C1s). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Complement Component 1, S Subcomponent (C1s) level in the sample or standard.
• ELISA Kit Range: 41.2-30,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	88-95	92
  EDTA Plasma (n=5)	78-101	95
  Heparin Plasma (n=5)	90-102	98

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Component 1, S Subcomponent (C1s) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Component 1, S Subcomponent (C1s) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • C1S Microplate: 96 Wells (12 x 8 Well strips)
  • C1S Lyophilized Standard: 2
  • 100X C1S Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.47 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: C1S
• Gene Full Name: complement C1s
• Alias Symbols: basic proline-rich peptide IB-1;C1 esterase;complement C1s subcomponent;complement component 1 subcomponent s;complement component 1, s subcomponent;EDSPD2.
• NCBI Gene Id: 716
• Protein Name: Complement C1s subcomponent
• Description of Target: C1s B chain is a serine protease that combines with C1q and C1r to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4.
• Uniprot ID: P09871
• Protein Accession #: NP_001333779
• Nucleotide Accession #: NM_001346850