Catalog No: OKCD03343
Size:96 Wells
Price: $800.00
SKU
OKCD03343
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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C1R Chemi-Luminescent ELISA Kit (Human) (OKCD03343)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodChemiluminescent, visible wavelength light
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)82-101%95-102%93-104%93-101%
EDTA Plasma (n=5)85-99%79-93%80-89%86-94%
Heparin Plasma (n=5)84-92%85-98%88-102%89-96%
ELISA Kit PrincipleAviva Systems Biology C1R Chemi-Luminescent ELISA Kit (Human) (OKCD03343) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C1R has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C1R is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample C1R
ELISA Kit Range0.078 - 5 ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)90-9895
EDTA Plasma (n=5)83-9786
Heparin Plasma (n=5)80-10184
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level C1R. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level C1R using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
C1R Microplate96 Wells (12 x 8 Well strips)
C1R Lyophilized Standard2
100X C1R Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.078 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolC1R
Alias SymbolsEDSPD1
NCBI Gene Id715
Protein NameComplement C1r subcomponent
Description of TargetC1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system.
Swissprot IdP00736
Protein Accession #NP_001724
Nucleotide Accession #NM_001733
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express C1R.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express C1R.
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