C1R Chemi-Luminescent ELISA Kit (Human) (OKCD03343)

Catalog No: OKCD03343

Size:96WELLS

Human C1R ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	82-101%	95-102%	93-104%	93-101%
  EDTA Plasma (n=5)	85-99%	79-93%	80-89%	86-94%
  Heparin Plasma (n=5)	84-92%	85-98%	88-102%	89-96%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Complement Component 1, R Subcomponent (C1r). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Complement Component 1, R Subcomponent (C1r). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Complement Component 1, R Subcomponent (C1r) level in the sample or standard.
• ELISA Kit Range: 0.41-300ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	90-98	95
  EDTA Plasma (n=5)	83-97	86
  Heparin Plasma (n=5)	80-101	84

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Component 1, R Subcomponent (C1r) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Component 1, R Subcomponent (C1r) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • C1R Microplate: 96 Wells (12 x 8 Well strips)
  • C1R Lyophilized Standard: 2
  • 100X C1R Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.078 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: C1R
• Gene Full Name: complement C1r
• Alias Symbols: complement C1r subcomponent;Complement component 1 subcomponent r;complement component 1, r subcomponent;EDSPD1.
• NCBI Gene Id: 715
• Protein Name: Complement C1r subcomponent
• Description of Target: C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system.
• Uniprot ID: P00736
• Protein Accession #: NP_001724
• Nucleotide Accession #: NM_001733