C1QTNF9 ELISA Kit (Human) (OKCD02409)

Catalog No: OKCD02409

Size:96WELLS

Human C1QTNF9 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	97-105%	79-98%	99-105%	98-105%
  EDTA Plasma (n=5)	95-105%	97-105%	87-101%	97-105%
  Heparin Plasma (n=5)	88-96%	91-98%	84-96%	95-102%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	83-101	88
  EDTA Plasma (n=5)	94-101	98
  Heparin Plasma(n=5)	88-103	94

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-C1QTNF9 Microplate: 96 Wells (12 x 8 Well strips)
  • C1QTNF9 Lyophilized Standard: 2
  • 100X Biotinylated C1QTNF9 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 6.3 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9). No significant cross-reactivity or interference between C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: C1QTNF9
• Gene Full Name: C1q and TNF related 9
• Alias Symbols: AQL1;C1q and tumor necrosis factor related protein 9;C1QTNF9A;complement C1q and tumor necrosis factor-related protein 9;complement C1q and tumor necrosis factor-related protein 9A;complement C1q tumor necrosis factor-related protein 9;complement C1q tumor necrosis factor-related protein 9A;CTRP9.
• NCBI Gene Id: 338872
• Protein Name: Complement C1q and tumor necrosis factor-related protein 9A
• Description of Target: Probable adipokine. Activates AMPK, AKT, and p44/42 MAPK signaling pathways.
• Uniprot ID: P0C862
• Protein Accession #: NP_001290066.1
• Nucleotide Accession #: NM_001303137.1
• Protein Size (# AA): 333