C1QTNF2 Antibody (OAPB00373)

Catalog No: OAPB00373 (Formerly GWB-D3DC01)

Size:100UG

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Western blot of recombinant CTRP2: (A) 5 ng, (B) 25 ng, and (C) 50 ng with CTRP2 at 1 μg/mL.

Immunofluorescence of CTRP2 in Jurkat cells with CTRP2 antibody at 20 μg/mL.

Immunocytochemistry of CTRP2 in Jurkat cells with CTRP2 antibody at 10 μg/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. CTRP2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Western blot
• Additional Information: Product Description: Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines. One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-alpha-related proteins (CTRPs) 1-7. Unlike adiponectin, which is expressed exclusively by differentiated adipocytes, the CTRPs are are expressed in a wide variety of tissues. These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene. Of the CTRPs, CTRP2 is most similar structurally and functionally to adiponectin. Recombinant CTRP2 rapidly activated AMPK and MAPK in cultured C2C12 cells, leading to increased glycogen accumulation and fatty acid oxidation.
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: CTRP2 antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: CTRP2 antibody was raised against recombinant human CTRP2.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 1 mg/ml
• Application Info: CTRP2 antibody can be used for the detection of CTRP2 by Western blot at 1 ug/ml. Caco-2 cell lysate can be used as positive control. Anti-CTRP2 is human, mouse, and rat reactive. These proteins are often highly modified post-translationally and migrate in SDS-PAGE at positions other than their predicted size. (Optimal dilution should be determined by user)
• Reference: Fantuzzi G. Adipose tissue, adipokines, and inflammation. J. Allergy Clin. Immunol. 2005; 115:911-9.;Tsao T-S, Lodish HF, and Fruebis J. ACRP30, a new hormone controlling fat and glucose metabolism. Euro. J. Pharmacol. 2002; 440:213-21.;Wong GW, Wang J, Hug C, et al. A family of Acrp30/ adiponectin structural and functional paralogs. Proc. Natl. Acad. Sci. USA 2004; 101:10302-7.;Shapiro L and Scherer PE. The crystal structure of a complement-1q family protein suggests an evolutionary link to tumor necrosis factor. Curr. Biol. 1998; 8:335-8.

Target Info

• Gene Symbol: C1QTNF2
• Gene Full Name: C1q and TNF related 2
• Alias Symbols: C1q and tumor necrosis factor related protein 2;complement C1q tumor necrosis factor-related protein 2;complement-c1q tumor necrosis factor-related protein 2;CTRP2;zacrp2.
• NCBI Gene Id: 114898
• Protein Name: Complement C1q tumor necrosis factor-related protein 2|complement C1q tumor necrosis factor-related protein 2 [Homo sapiens]
• Description of Target: a novel adipokine
• Uniprot ID: Q9BXJ5
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_114114
• Nucleotide Accession #: NM_031908.4