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Catalog No: OKCD08724
Size:96WELLS
Price: $800.00
SKU
OKCD08724
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Datasheets/ManualsPrintable datasheet for C1QC ELISA Kit (Human) (OKCD08724)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 1, Q Subcomponent C (C1qC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 1, Q Subcomponent C (C1qC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 1, Q Subcomponent C (C1qC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Complement Component 1, Q Subcomponent C (C1qC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range125-8,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Component 1, Q Subcomponent C (C1qC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Component 1, Q Subcomponent C (C1qC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
C1QC Microplate96 Wells (12 x 8 Well strips)
C1QC Lyophilized Standard2
100X C1QC HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids.
Sensitivity< 13.7pg/mL
Gene SymbolC1QC
Gene Full Namecomplement C1q C chain
Alias SymbolsC1Q-C;C1QG;complement C1q subcomponent subunit C;complement component 1, q subcomponent, C chain;complement component 1, q subcomponent, gamma polypeptide.
NCBI Gene Id714
Protein NameComplement C1q subcomponent subunit C
Description of TargetC1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
Uniprot IDP02747
Protein Accession #NP_001107573.1
Nucleotide Accession #NM_001114101.2
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