C1QC Chemi-Luminescent ELISA Kit (Human) (OKCD03342)

Catalog No: OKCD03342

Size:96WELLS

Human C1QC ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	80-105%	90-104%	95-104%	93-101%
  EDTA Plasma (n=5)	79-96%	78-89%	85-103%	96-103%
  Heparin Plasma (n=5)	94-101%	93-101%	99-105%	79-96%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Complement Component 1, Q Subcomponent C (C1qC). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Complement Component 1, Q Subcomponent C (C1qC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Complement Component 1, Q Subcomponent C (C1qC) level in the sample or standard.
• ELISA Kit Range: 2.74-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	82-101	91
  EDTA Plasma (n=5)	89-97	94
  Heparin Plasma (n=5)	80-95	91

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Component 1, Q Subcomponent C (C1qC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Component 1, Q Subcomponent C (C1qC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • C1QC Microplate: 96 Wells (12 x 8 Well strips)
  • C1QC Lyophilized Standard: 2
  • 100X C1QC Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 31.2 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: C1QC
• Gene Full Name: complement C1q C chain
• Alias Symbols: C1Q-C;C1QG;complement C1q subcomponent subunit C;complement component 1, q subcomponent, C chain;complement component 1, q subcomponent, gamma polypeptide.
• NCBI Gene Id: 714
• Protein Name: Complement C1q subcomponent subunit C
• Description of Target: C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
• Uniprot ID: P02747
• Protein Accession #: NP_001107573
• Nucleotide Accession #: NM_001114101