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C1qb ELISA Kit (Rat) (OKCD01745)

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Gene Symbol:
C1QB
Official Gene Full Name:
Complement C1q B chain
NCBI Gene Id:
29687
Alias Symbols:
Complement C1q subcomponent subunit B
Description of Target:
C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca2+-dependent C1r2C1s2 proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
Protein Name:
Complement C1q subcomponent subunit B
Swissprot Id:
P31721
Protein Accession #:
NP_062135.1
Nucleotide Accession #:
NM_019262.1
Sample Type:
Serum, plasma and other biological fluids
Sensitivity:
< 0.64 ng/mL
Kit Range:
1.56 - 100 ng/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level C1qb was tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level C1qb was tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
Kit Duration:
~3.5 Hours
Kit Principle:
Aviva Systems Biology C1qb ELISA Kit (Rat) (OKCD01745) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C1qb has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C1qb is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution.The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample C1qb captured in the well.
Kit Component:
ComponentAmount
Anti-C1qb Microplate96 Wells (12 x 8 Well Strips)
C1qb Lyophilized Standard2 x 100 ng
100X Biotinylated C1qb Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Linearity:
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)95-103%81-102%96-104%95-103%
EDTA Plasma (n=5)85-103%91-102%82-97%84-92%
Heparin Plasma (n=5)98-105%95-105%86-101%96-103%
Kit Recovery:
MatrixRecovery Range (%)Average (%)
Serum (n=5)78-10492
EDTA Plasma (n=5)96-105102
Heparin Plasma (n=5)79-9484
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express C1QB.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express C1QB.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay has high sensitivity and excellent specificity for detection of Complement Component 1, Q Subcomponent B (C1qB). No significant cross-reactivity or interference between Complement Component 1, Q Subcomponent B (C1qB) and analogues was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Protein Size (# AA):
253

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