C1QA ELISA Kit (Bovine) (OKEH03869)

Catalog No: OKEH03869

Size:96T

Bovine C1QA ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology C1QA ELISA Kit (Bovine) (OKEH03869) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C1QA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C1QA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample C1QA captured in well.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Component:
  • Anti-C1QA Microplate: 96 Wells (12 x 8 Well strips)
  • C1QA Lyophilized Standard: 2
  • 100X Biotinylated C1QA Detector Antibody: 1 x 120 ÂuL
  • 100X Avidin-HRP Conjugate: 1 x 120 ÂuL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.53 ng/mL
• Specificity: Natural and recombinant Bovine Complement C1q subcomponent subunit A
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: C1QA
• Gene Full Name: complement C1q A chain
• Alias Symbols: complement C1q subcomponent subunit A;complement component 1, q subcomponent, alpha polypeptide.
• NCBI Gene Id: 534961
• Protein Name: Q5E9E3
• Description of Target: Complement C1q subcomponent subunit A
• Uniprot ID: https://www.uniprot.org/uniprot/Q5E9E3
• Protein Accession #: NP_001014945.1
• Nucleotide Accession #: NM_001014945.2
• Protein Size (# AA): 244
• Molecular Weight: 26 kDa