C1q ELISA Kit (Mouse) (OKIA00220)

Catalog No: OKIA00220

Size:1 x 96 well plate

Representative Mouse C1q Standard Curve plotted with four parameter logistic regression (4PL). Concentration is plotted as ng/mL.

Product Info

• Predicted Species Reactivity: Mouse
• Product Format: 12 of 8-well strips
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: 100 Minutes
• ELISA Kit Principle: The Aviva Systems Biology C1q ELISA Kit (Mouse) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Complement C1q (whole molecule) in Plasma or Serum. This assay employs an antibody specific for Mouse C1q coated on a 96-well plate. Standards and samples are pipetted into the wells and Complement C1q (whole molecule) present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-C1q antibodies conjugated with horseradish peroxidase (HRP) are added. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of C1q bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The quantity of C1q in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.
• ELISA Kit Range: 1.57 - 100 ng/mL
• ELISA Kit Reproducibility: < 10%
• ELISA Kit Component:
  • Anti-Mouse C1q Microplate: 96 Wells (12 x 8 Well Strips)
  • Mouse C1q Calibrator: 1
  • 100X C1q-HRP Conjugate: 150 uL
  • 5X Assay Diluent: 50 mL
  • 20X Wash Solution: 50 mL
  • Chromogen-Substrate Solution: 12 mL
  • Stop Solution: 12 mL
• Additional Information: Intended Use: The C1q test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring C1q/Complement C1q in Mouse biological samples.
  Sample Volume: 5 uL
• Reconstitution and Storage: Store at 4 degrees
• Sensitivity: 0.45 ng/mL

Target Info

• Alias Symbols: C1q;Complement C1q;
• Protein Name: Complement C1q (whole molecule)
• Description of Target: The complement component 1q (or simply C1q) is a protein complex involved in the complement system, which is part of the innate immune system. C1q together with C1r and C1s form the C1 complex.