C-raf Antibody (OAPB01117)

Catalog No: OAPB01117

Size:0.1 mg

Western blot analysis of C-raf in 3T3 cell lysate with C-raf antibody at (A) 0.5 and (B) 1 μg/mL.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Host: chicken
• Application: WB
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: C-raf antibody can be stored at 4C, stable for three months. Store at -20C for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: The immunogen for anti-C-raf antibody: antibody was raised against an 19 amino acid peptide near the amino terminus of human C-raf.
• Purification: Affinity chromatography purified via peptide column
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Blocking Peptide: For anti-C-raf antibody is Catalog # OEPB03233
• Application Info: C-raf antibody can be used for detection of C-raf by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)
• Reference: Howe LR, Leevers SJ, Gomez N, et al. Activation of the MAP kinase pathway by the protein kinase raf. Cell1992; 71:335-42.;Chang F, Steelman LS, Shelton JG, et al. Regulation of cell cycle progression and apoptosis by the Ras/Raf/MEK/ERK pathway. Int. J. Oncol.2003; 22:469-80.;Pandit B, Sarkozy A, Pennacchio LA, et al. Gain-of-function RAF1 mutations cause Noonan and LEOPARD syndromes with hypertrophic cardiomyopathy. Nat. Genet.2007; 39:1007-12.;

Target Info

• Gene Symbol: RAF1
• Alias Symbols: NS5, CRAF, Raf-1, c-Raf, CMD1NN
• NCBI Gene Id: 5894
• Protein Name: RAF proto-oncogene serine/threonine-protein kinase
• Description of Target: v-raf murine sarcoma viral oncogene homolog 1
• Uniprot ID: P04049