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Datasheets/Manuals | Printable datasheet for C-raf Antibody (OAPB01117) (OAPB01117) |
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Predicted Species Reactivity | Bovine|Chicken|Human|Mouse|Rat |
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Product Format | Liquid. C-raf Antibody is supplied in PBS containing 0.02% sodium azide. |
Clonality | Polyclonal |
Host | chicken |
Conjugation | Unconjugated |
Application | Enzyme-linked immunosorbent assay|Western blot |
:: | Buffer: Antibody is supplied in PBS containing 0.02% sodium azide. |
Reconstitution and Storage | C-raf antibody can be stored at 4C, stable for three months. Store at -20C for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Immunogen | C-raf antibody was raised against an 19 amino acid synthetic peptide near the amino terminus of human C-raf. The immunogen is located within amino acids 30 - 80 of C-raf. |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Human; Mouse; Rat |
Concentration | 1 mg/ml |
Blocking Peptide | For anti-C-raf antibody is Catalog # OEPB03233 |
Application Info | C-raf antibody can be used for detection of C-raf by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user) |
Reference | Howe LR, Leevers SJ, Gomez N, et al. Activation of the MAP kinase pathway by the protein kinase raf. Cell1992; 71:335-42.;Chang F, Steelman LS, Shelton JG, et al. Regulation of cell cycle progression and apoptosis by the Ras/Raf/MEK/ERK pathway. Int. J. Oncol.2003; 22:469-80.;Pandit B, Sarkozy A, Pennacchio LA, et al. Gain-of-function RAF1 mutations cause Noonan and LEOPARD syndromes with hypertrophic cardiomyopathy. Nat. Genet.2007; 39:1007-12.; |
Gene Symbol | RAF1 |
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Gene Full Name | Raf-1 proto-oncogene, serine/threonine kinase |
Alias Symbols | CMD1NN;c-Raf;CRAF;C-Raf proto-oncogene, serine/threonine kinase;NS5;Oncogene RAF1;proto-oncogene c-RAF;raf proto-oncogene serine/threonine protein kinase;RAF proto-oncogene serine/threonine-protein kinase;Raf-1;v-raf-1 murine leukemia viral oncogene homolog 1;v-raf-1 murine leukemia viral oncogene-like protein 1. |
NCBI Gene Id | 5894 |
Protein Name | RAF proto-oncogene serine/threonine-protein kinase|RAF proto-oncogene serine/threonine-protein kinase isoform b [Homo sapiens] |
Description of Target | Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation. |
Uniprot ID | P04049 |
Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/NP_002871 |
Molecular Weight | Predicted: 72 kDa Observed: 72 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "C-raf Antibody (OAPB01117)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Bovine|Chicken|Human|Mouse|Rat".
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How long will it take to receive "C-raf Antibody (OAPB01117)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "C-raf Antibody (OAPB01117)" provided in?
This item is provided in "Liquid. C-raf Antibody is supplied in PBS containing 0.02% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "C-raf Antibody (OAPB01117)"?
This target may also be called "CMD1NN;c-Raf;CRAF;C-Raf proto-oncogene, serine/threonine kinase;NS5;Oncogene RAF1;proto-oncogene c-RAF;raf proto-oncogene serine/threonine protein kinase;RAF proto-oncogene serine/threonine-protein kinase;Raf-1;v-raf-1 murine leukemia viral oncogene homolog 1;v-raf-1 murine leukemia viral oncogene-like protein 1." in publications.
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What is the shipping cost for "C-raf Antibody (OAPB01117)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "C-raf Antibody (OAPB01117)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "C-raf Antibody (OAPB01117)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "Predicted: 72 kDa Observed: 72 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "C-raf Antibody (OAPB01117)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "RAF1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "RAF1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "RAF1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "RAF1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "RAF1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "RAF1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.