C-raf Antibody : HRP (OAPB01117-HRP)

Catalog No: OAPB01117-HRP

Size:0.1 mg

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
• Clonality: Polyclonal
• Host: chicken
• Conjugation: HRP: Horseradish Peroxidase
• Application: ELISA, WB
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: The immunogen for anti-C-raf antibody: antibody was raised against an 19 amino acid peptide near the amino terminus of human C-raf.
• Purification: Affinity chromatography purified via peptide column
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 0.6-0.7 mg/ml
• Blocking Peptide: For anti-C-raf antibody is Catalog # OEPB03233
• Application Info: C-raf antibody can be used for detection of C-raf by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)
• Reference: Howe LR, Leevers SJ, Gomez N, et al. Activation of the MAP kinase pathway by the protein kinase raf. Cell1992; 71:335-42.;Chang F, Steelman LS, Shelton JG, et al. Regulation of cell cycle progression and apoptosis by the Ras/Raf/MEK/ERK pathway. Int. J. Oncol.2003; 22:469-80.;Pandit B, Sarkozy A, Pennacchio LA, et al. Gain-of-function RAF1 mutations cause Noonan and LEOPARD syndromes with hypertrophic cardiomyopathy. Nat. Genet.2007; 39:1007-12.;

Target Info

• Gene Symbol: RAF1
• Alias Symbols: NS5, CRAF, Raf-1, c-Raf, CMD1NN
• NCBI Gene Id: 5894
• Protein Name: RAF proto-oncogene serine/threonine-protein kinase
• Description of Target: v-raf murine sarcoma viral oncogene homolog 1
• Uniprot ID: P04049