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c-Jun (Phospho-Thr93) DNA-Binding ELISA Kit (OKAG00291)

Catalog#: OKAG00291
Domestic: within 1 week delivery | International: 1 week
More Information
Predicted Species ReactivityHuman, Mouse, Rat
Product Format12 x 8-Well Microstrips
Kit Detection MethodColorimetric, OD450 nm
Kit PrincipleThe Aviva DNA-Binding ELISA Kit contains components necessary for detection of active transcription factors in eukaryotic nuclear or cell lysates. This particular immunoassay utilizes the qualitative technique of an indirect ELISA. Streptavidin is bound to the immunoassay plate and specific biotinylated double-stranded (dsDNA) oligonucleotides are then added to bind to the streptavidin via a high affinity biotin-streptavidin interaction. After subsequent blocking of extraneous binding sites in each well, the sample containing the target of interest can be added. Primary antibody is added to bind activated transcription factors bound to the dsDNA oligonucleotide, which has been immobilized via the plate-coated streptavidin. A HRP-conjugated secondary antibody specific for rabbit IgGs is added, which allows for specific binding to the Primary Antibody, and consequently colorimetric detection upon addition of the TMB substrate.
For color development, TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added to each well. After addition of the substrate, a peroxidase catalyzed reaction will produce a blue TMB Diimine product that is proportional to the target concentration in the sample. Color development is quenched by addition of Stop Solution, or 2 N Sulfuric Acid, which turns the solution yellow. The absorbance can then be read by a spectrophotometer at 450 nm and subsequently allowing for determination of the target concentration in the sample.
Kit Component
dsDNA Oligonucleotide Coated Microplate96 Wells (12 x 8 Strips)Microstrips
100X Primary Antibody100 uL
100X Primary Phospho-Antibody100 uL
HRP-Conjugated Anti-Rabbit IgG Antibody2 x 6 mL
Nuclear Lysate Positive ControlLyophilized
Wild-Type Consensus dsDNA Oligonucleotide20 uL
Mutant Consensus dsDNA Oligonucleotide20 uL
10X Wash Buffer50 mL
2X Binding Buffer12 mL
Primary Antibody Diluent12 mL
100X Protease and Phosphatase Inhibitors100 uL
Nuclear Wash Buffer12 mL
Cytoplasmic Extraction Buffer6 mL
Nuclear Extraction Buffer6 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
::OMIM: 165160
UniGene: Hs.714791
::Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.
Reconstitution and StorageStore as indicated in product manual.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
SpecificityThe c-Jun (Phospho-Thr93) DNA-Binding ELISA Kit detects endogenous levels of c-Jun only when phosphorylated at Thr93.
Assay InfoAssay Type: Qualitative DNA Binding ELISA
Gene SymbolJUN
Official Gene Full NameJun proto-oncogene, AP-1 transcription factor subunit
Alias SymbolsActivator protein 1, AP1, AP-1, c-Jun, p39, Proto-oncogene c-Jun, Transcription factor AP-1, V-jun avian sarcoma virus 17 oncogene homolog
NCBI Gene Id3725
Protein Nametranscription factor AP-1
Description of TargetThis gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.
Swissprot IdP05412
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express JUN.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express JUN.
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