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Catalog No: OCOA12979
Size:20UG
Price: $179.00
SKU
OCOA12979
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BUB3 Protein Lysate (OCOA12979)

Datasheets/ManualsPrintable datasheet for BUB3 Protein Lysate (OCOA12979)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionBUB3 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolBUB3
Gene Full NameBUB3 mitotic checkpoint protein
Alias SymbolsBUB3 budding uninhibited by benzimidazoles 3 homolog;BUB3L;budding uninhibited by benomyl;budding uninhibited by benzimidazoles 3 homolog;hBUB3;mitotic checkpoint component;mitotic checkpoint protein BUB3;testicular tissue protein Li 27.
NCBI Gene Id9184
Protein NameNM_004725, NP_004716, O43684
Description of TargetHomo sapiens BUB3, mitotic checkpoint protein (BUB3), transcript variant 1, mRNA, Mitotic checkpoint protein BUB3, mitotic checkpoint protein BUB3 isoform a [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/O43684
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_004716
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_004725
Molecular Weight37 kDa
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