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Datasheets/Manuals | Printable datasheet for BUB1B Antibody (OAAF02041) |
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Predicted Species Reactivity | Human|Mouse |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Western blot |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human BUB1B. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity | BUB1B antibody detects endogenous levels of total BUB1B protein. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | WB: 1:500~1:3000; ELISA: 1:20000 |
Reference | Taylor S.S., J. Cell Biol. 142:1-11(1998). Chan G.K.T., J. Cell Biol. 143:49-63(1998). Cahill D.P., Nature 392:300-303(1998). |
Gene Symbol | BUB1B |
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Gene Full Name | BUB1 mitotic checkpoint serine/threonine kinase B |
Alias Symbols | Bub1A;BUB1B, mitotic checkpoint serine/threonine kinase;BUB1beta;BUBR1;budding uninhibited by benzimidazoles 1 homolog beta;hBUBR1;MAD3/BUB1-related protein kinase;MAD3L;mitotic checkpoint kinase MAD3L;mitotic checkpoint serine/threonine-protein kinase BUB1 beta;MVA1;Protein SSK1;SSK1. |
NCBI Gene Id | 701 |
Protein Name | Mitotic checkpoint serine/threonine-protein kinase BUB1 beta |
Description of Target | Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression. |
Uniprot ID | O60566 |
Protein Accession # | NP_001202.4 |
Molecular Weight | 119 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "BUB1B Antibody (OAAF02041)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse".
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How long will it take to receive "BUB1B Antibody (OAAF02041)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "BUB1B Antibody (OAAF02041)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "BUB1B Antibody (OAAF02041)"?
This target may also be called "Bub1A;BUB1B, mitotic checkpoint serine/threonine kinase;BUB1beta;BUBR1;budding uninhibited by benzimidazoles 1 homolog beta;hBUBR1;MAD3/BUB1-related protein kinase;MAD3L;mitotic checkpoint kinase MAD3L;mitotic checkpoint serine/threonine-protein kinase BUB1 beta;MVA1;Protein SSK1;SSK1." in publications.
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What is the shipping cost for "BUB1B Antibody (OAAF02041)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "BUB1B Antibody (OAAF02041)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "BUB1B Antibody (OAAF02041)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "119 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "BUB1B Antibody (OAAF02041)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "BUB1B"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "BUB1B"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "BUB1B"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "BUB1B"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "BUB1B"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "BUB1B"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.