BUB1 Antibody (OAAF02028)

Catalog No: OAAF02028 (Formerly GWB-ASD301)

Size:100 ug

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using BUB1 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human BUB1.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: BUB1 antibody detects endogenous levels of total BUB1 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: IHC: 1:50~1:100; ELISA: 1:10000
• Reference: Cahill D.P., Nature 392:300-303(1998).
  Ouyang B., Cell Growth Differ. 9:877-885(1998).
  Taylor S.S., J. Cell Biol. 142:1-11(1998).

Target Info

• Gene Symbol: BUB1
• Gene Full Name: BUB1 mitotic checkpoint serine/threonine kinase
• Alias Symbols: BUB1 budding uninhibited by benzimidazoles 1 homolog;BUB1A;BUB1L;budding uninhibited by benzimidazoles 1 homolog;hBUB1;mitotic checkpoint serine/threonine-protein kinase BUB1;mitotic spindle checkpoint kinase;putative serine/threonine-protein kinase.
• NCBI Gene Id: 699
• Protein Name: Mitotic checkpoint serine/threonine-protein kinase BUB1
• Description of Target: Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Required for centromeric enrichment of AUKRB in prometaphase. Plays an important role in defining SGO1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis.
• Uniprot ID: O43683
• Protein Accession #: NP_004327.1
• Molecular Weight: 122 kDa