BTN1A1 ELISA Kit (Human) (OKCD08096)

Catalog No: OKCD08096

Size:96 Wells

BTN1A1 ELISA Kit (Human) (OKCD08096) Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology BTN1A1 ELISA Kit (Human) (OKCD08096) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BTN1A1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BTN1A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BTN1A1 captured in the well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level BTN1A1. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level BTN1A1 using 8 replicates on each of 3 plates.
  Intra-Assay CV <10%
  Inter-Assay CV <12%
• ELISA Kit Component:
  • BTN1A1 Microplate: 96 Wells (12 x 8 Well strips)
  • BTN1A1 Lyophilized Standard: 2
  • 100X BTN1A1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.057ng/mL

Target Info

• Gene Symbol: BTN1A1
• Gene Full Name: butyrophilin subfamily 1 member A1
• Alias Symbols: BT, BTN, BTN1
• NCBI Gene Id: 696
• Protein Name: Butyrophilin subfamily 1 member A1
• Description of Target: Butyrophilin is the major protein associated with fat droplets in the milk. It is a member of the immunoglobulin superfamily. It may have a cell surface receptor function. The human butyrophilin gene is localized in the major histocompatibility complex (MHC) class I region of 6p and may have arisen relatively recently in evolution by the shuffling of exons between 2 ancestral gene families.Butyrophilin is the major protein associated with fat droplets in the milk. It is a member of the immunoglobulin superfamily. It may have a cell surface receptor function. The human butyrophilin gene is localized in the major histocompatibility complex (MHC) class I region of 6p and may have arisen relatively recently in evolution by the shuffling of exons between 2 ancestral gene families.
• Uniprot ID: Q13410
• Protein Accession #: NP_001723.2
• Nucleotide Accession #: NM_001732.2