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Catalog No: OCOA01841
Size:20UG
Price: $179.00
SKU
OCOA01841
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BTG2 Protein Lysate (OCOA01841)

Datasheets/ManualsPrintable datasheet for BTG2 Protein Lysate (OCOA01841)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionBTG2 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolBTG2
Gene Full NameBTG anti-proliferation factor 2
Alias SymbolsAPRO1;B-cell translocation gene 2;BTG family member 2;nerve growth factor-inducible anti-proliferative;NGF-inducible anti-proliferative protein PC3;PC3;pheochromacytoma cell-3;protein BTG2;TIS21.
NCBI Gene Id7832
Protein NameNM_006763, NP_006754, P78543
Description of TargetHomo sapiens BTG anti-proliferation factor 2 (BTG2), mRNA, Protein BTG2, protein BTG2 [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/P78543
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_006754
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_006763
Molecular Weight17.2 kDa
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