BSG Antibody (OAEE00091)

Catalog No: OAEE00091

Size:100UG

Surface staining of human peripheral blood with anti-human CD147 (MEM-M6/1) biotin.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: MEM-M6/1
• Isotype: IgG1
• Host: Mouse
• Conjugation: Biotin
• Application: Flow cytometry|Immunohistochemistry-Paraffin|Immunoprecipitation|Western blot
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: Protein A-CR purified soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the hinge region, CH2-and CH3 domain of human IgG1 (CD147Rg is secreted by transfectants as a dimer).
• Purification: Purified by size-exclusion chromatography
• Concentration: 1 mg/ml
• Specificity: The antibody MEM-M6/1 recognizes an epitope in the N-terminal Ig domain (D1) of CD147 (Neurothelin), a 50-60 kDa type I transmembrane glycoprotein primarily expressed on all leukocytes, red blood cells, platelets and endothelial cells; it is not expressed by resting lymphocytes.
  The antibody MEM-M6/1 is a high-affinity antibody capable of binding to unstimulated peripheral blood T cells.
• Formulation: Biotin
• Storage Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
• Reference: Koch C, Staffler G, Huttinger R, Hilgert I, Prager E, Cerny J, Steinlein P, Majdic O, Horejsi V, Stockinger H: T cell activation-associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density. Int Immunol. 1999 May;11(5):777-86.
  Schmidt R, Bültmann A, Fischel S, Gillitzer A, Cullen P, Walch A, Jost P, Ungerer M, Tolley ND, Lindemann S, Gawaz M, Schömig A, May AE. Extracellular matrix metalloproteinase inducer (CD147) is a novel receptor onplatelets, activates platelets, and augments nuclear factor kappaB-dependent inflammation in monocytes. Circ Res. 2008 Feb 15;102(3):302-9.
  Melchior A, Denys A, Deligny A, Mazurier J, Allain F: Cyclophilin B induces integrin-mediated cell adhesion by a mechanism involving CD98-dependent activation of protein kinase C-delta and p44/42 mitogen-activatedprotein kinases. Exp Cell Res. 2008 Feb 1;314(3):616-28.
  Ruiz S, Castro-Castro A, Bustelo XR: CD147 Inhibits the Nuclear Factor of Activated T-cells by Impairing Vav1 and Rac1 Downstream Signaling. J Biol Chem. 2008 Feb 29;283(9):5554-66.
  Iacono KT, Brown AL, Greene MI, Saouaf SJ: CD147 immunoglobulin superfamily receptor function and role in pathology. Exp Mol Pathol. 2007 Dec;83(3):283-95.
  Xu D, Hemler ME: Metabolic activation-related CD147-CD98 complex. Mol Cell Proteomics. 2005 Aug;4(8):1061-71.
  Wilson MC, Meredith D, Fox JE, Manoharan C, Davies AJ, Halestrap AP: Basigin (CD147) is the target for organomercurial inhibition of monocarboxylatetransporter isoforms 1 and 4: the ancillary protein for the insensitive MCT2 is EMBIGIN (gp70). J Biol Chem. 2005 Jul 22;280(29):27213-21.
  Kirk P, Wilson MC, Heddle C, Brown MH, Barclay AN, Halestrap AP: CD147 is tightly associated with lactate transporters MCT1 and MCT4 and facilitates their cell surface expression. EMBO J. 2000 Aug 1;19(15):3896-904.

Target Info

• Gene Symbol: BSG
• Gene Full Name: basigin (Ok blood group)
• Alias Symbols: 5F7;basigin;CD147;collagenase stimulatory factor;EMMPRIN;EMPRIN;extracellular matrix metalloproteinase inducer;HAb18G;hepatoma-associated antigen;leukocyte activation antigen M6;OK;OK blood group antigen;SLC7A11;TCSF;tumor cell-derived collagenase stimulatory factor.
• NCBI Gene Id: 682
• Protein Name: Basigin
• Description of Target: Plays an important role in targeting the monocarboxylate transporters SLC16A1, SLC16A3, SLC16A8 and SLC16A11 to the plasma membrane. Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.
• Uniprot ID: P35613
• Protein Accession #: NP_001719.2; NP_940991.1; NP_940992.1; NP_940993.1; XP_005259676.1
• Nucleotide Accession #: NM_001728.3; NM_198589.2; NM_198590.2; NM_198591.2; XM_005259619.1