BRSK2 Antibody (OAPB00510)

Catalog No: OAPB00510

Size:100UG

Western blot analysis of BRSK2 in human brain tissue lysate with BRSK2 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.

Immunohistochemistry of BRSK2 in human brain tissue with BRSK2 antibody at 5 ug/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. BRSK2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: BRSK2 antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: BRSK2 antibody was raised against a 23 amino acid synthetic peptide from near the carboxy terminus of human BRSK2. The immunogen is located within amino acids 630 - 680 of BRSK2.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-BRSK2 (OAPB00510) antibody is Catalog # OEPB02627
• Application Info: BRSK2 antibody can be used for detection of BRSK2 by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)
• Reference: Manning G, Whyte DB, Martinez R, et al. The protein kinase complement of the human genome. Science2002; 298:1912-34.;Lizcano JM, Goransson O, Toth R, et al. LKB1 is a master kinase that activates 13 kinases of the AMPK subfamily, including MARK/PAR-1. EMBO J.2004; 23:833-43.;Kishi M, Pan YA, Crump J, et al. Mammalian SAD kinases are required for neuronal polarization. Science2005; 307:929-32.;Sabater L, Gomez-Choco M, Saiz A, et al. BR serine/threonine kinase 2: A new autoantigen in paraneoplastic limbic encephalitis. J. Neuroimmunol.2005; 170:186-90.

Target Info

• Gene Symbol: BRSK2
• Gene Full Name: BR serine/threonine kinase 2
• Alias Symbols: BR serine/threonine-protein kinase 2;brain-selective kinase 2;brain-specific serine/threonine-protein kinase 2;C11orf7;PEN11B;protein kinase SAD1B;SAD1;SADA;serine/threonine-protein kinase 29;serine/threonine-protein kinase BRSK2;serine/threonine-protein kinase SAD-A;STK29;testicular tissue protein Li 30.
• NCBI Gene Id: 9024
• Protein Name: Serine/threonine-protein kinase BRSK2
• Description of Target: Serine/threonine-protein kinase that plays a key role in polarization of neurons and axonogenesis, cell cycle progress and insulin secretion. Phosphorylates CDK16, CDC25C, MAPT/TAU, PAK1 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in postmitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. Plays a role in the regulation of the mitotic cell cycle progress and the onset of mitosis. Plays a role in the regulation of insulin secretion in response to elevated glucose levels, probably via phosphorylation of CDK16 and PAK1. While BRSK2 phosphorylated at Thr-174 can inhibit insulin secretion (PubMed:22798068), BRSK2 phosphorylated at Thr-260 can promote insulin secretion (PubMed:22669945). Regulates reorganization of the actin cytoskeleton. May play a role in the apoptotic response triggered by endoplasmic reticulum (ER) stress.
• Uniprot ID: Q8IWQ3
• Protein Accession #: NP_001243556.1; NP_001243558.1; NP_001243559.1; NP_001269147.1; NP_003948.2
• Nucleotide Accession #: NM_001256627.1; NM_001256629.1; NM_001256630.1; NM_001282218.1; NM_003957.3