BRSK1 Antibody (OAPB00508)

Catalog No: OAPB00508

Size:100UG

Western blot analysis of BRSK1 in human brain tissue lysate with BRSK1 antibody at (A) 0.5 and (B) 1 ug/mL.

Immunohistochemistry of BRSK1 in human brain tissue with BRSK1 antibody at 2.5 ug/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. BRSK1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: BRSK1 antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: BRSK1 antibody was raised against a 28 amino acid synthetic peptide from near the carboxy terminus of human BRSK1. The immunogen is located within amino acids 720 - 770 of BRSK1.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-BRSK1 (OAPB00508) antibody is Catalog # OEPB02621
• Application Info: BRSK1 antibody can be used for detection of BRSK1 by Western blot at 0.5 - 1 ug/ml. (Optimal dilution should be determined by user)
• Reference: Lu R, Niida H, and Nakanishi M. Human SAD1 kinase is involved in UV-induced DNA damage checkpoint function. J. Biol. Chem.2004; 279:31164-70.;Kishi M, Pan YA, Crump J, et al. Mammalian SAD kinases are required for neuronal polarization. Science2005; 307:929-32.;

Target Info

• Gene Symbol: BRSK1
• Gene Full Name: BR serine/threonine kinase 1
• Alias Symbols: BR serine/threonine-protein kinase 1;brain-selective kinase 1;brain-specific serine/threonine-protein kinase 1;hSAD1;protein kinase SAD1A;SAD1 homolog;SAD1 kinase;serine/threonine-protein kinase BRSK1;serine/threonine-protein kinase SAD-B;synapses of Amphids Defective homolog 1.
• NCBI Gene Id: 84446
• Protein Name: Serine/threonine-protein kinase BRSK1
• Description of Target: Serine/threonine-protein kinase that plays a key role in polarization of neurons and centrosome duplication. Phosphorylates CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. In neurons, localizes to synaptic vesicles and plays a role in neurotransmitter release, possibly by phosphorylating RIMS1. Also acts as a positive regulator of centrosome duplication by mediating phosphorylation of gamma-tubulin (TUBG1 and TUBG2) at 'Ser-131', leading to translocation of gamma-tubulin and its associated proteins to the centrosome. Involved in the UV-induced DNA damage checkpoint response, probably by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C.
• Uniprot ID: Q8TDC3
• Protein Accession #: NP_115806.1
• Nucleotide Accession #: NM_032430.1