BRK1 ELISA Kit (Mouse) (OKEH08590)

Catalog No: OKEH08590

Size:96T

Mouse BRK1 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology BRK1 ELISA Kit (Mouse) (OKEH08590) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BRK1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BRK1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BRK1 captured in well.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=8.1% (n = 20)
  Mean Inter-assay CV%: <=9.4% (n = 20)
• ELISA Kit Component:
  • BRK1 Microplate: 96 Wells (12 x 8 Well strips)
  • BRK1 Lyophilized Standard: 2
  • 100X Biotinylated BRK1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Involved in regulation of actin and microtubule organization. Part of a WAVE complex that activates the Arp2/3 complex (By similarity). As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (PubMed:27605705).
  Subcellular Location: Cytoplasm Cytoskeleton
  Unigene: Mm.485793
  SMR: Q91VR8
  String: 10090.ENSMUSP00000041644
  KEGG: mmu:101314
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.43ng/mL
• Specificity: Natural and recombinant Mouse Protein BRICK1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: BRK1
• Gene Full Name: BRICK1, SCAR/WAVE actin-nucleating complex subunit
• Alias Symbols: AW011779, 6720456B07Rik
• NCBI Gene Id: 101314
• Protein Name: protein BRICK1
• Uniprot ID: Q91VR8
• Protein Accession #: NP_598698.1
• Nucleotide Accession #: NM_133937.1