Catalog No: OKEH08589
Size:96T
Price: $725.00
SKU
OKEH08589
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology BRK1 ELISA Kit (Human) (OKEH08589) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BRK1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BRK1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BRK1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 93% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=6.1% (n = 20)
Mean Inter-assay CV%: <=10.7% (n = 20)
ELISA Kit Component
ComponentAmount
BRK1 Microplate96 Wells (12 x 8 Well strips)
BRK1 Lyophilized Standard2
100X Biotinylated BRK1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Involved in regulation of actin and microtubule organization. Part of a WAVE complex that activates the Arp2/3 complex. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes.
::Subcellular Location: Cytoplasm Cytoskeleton
::Unigene: Hs.649307
SMR: Q8WUW1
String: 9606.ENSP00000432472
::KEGG: hsa:55845
MIM: 611183
Reconstitution and StorageStore as indicated in product manual.
Sensitivity0.089ng/mL
SpecificityNatural and recombinant Human Protein BRICK1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolBRK1
Gene Full NameBRICK1 subunit of SCAR/WAVE actin nucleating complex
Alias SymbolsBRICK1, SCAR/WAVE actin nucleating complex subunit;C3orf10;haematopoietic stem/progenitor cell protein 300;hHBrk1;HSPC300;MDS027;protein BRICK1.
NCBI Gene Id55845
Protein NameQ8WUW1
Description of TargetProtein BRICK1
Uniprot IDhttps://www.uniprot.org/uniprot/Q8WUW1
Protein Accession #NP_060932.2
Nucleotide Accession #NM_018462.4
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