Catalog No: OKCD01097 (Formerly GWB-KBBQT3)
Size:96WELLS
Price: $750.00
SKU
OKCD01097
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
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- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
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Datasheets/Manuals | Printable datasheet for BRINP3 ELISA Kit (Human) (OKCD01097) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Family With Sequence Similarity 5, Member C (FAM5C). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Family With Sequence Similarity 5, Member C (FAM5C). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Family With Sequence Similarity 5, Member C (FAM5C), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Family With Sequence Similarity 5, Member C (FAM5C) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Family With Sequence Similarity 5, Member C (FAM5C) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Family With Sequence Similarity 5, Member C (FAM5C) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Serum, plasma, tissue homogenates and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 0.116 ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of Family With Sequence Similarity 5, Member C (FAM5C). No significant cross-reactivity or interference between Family With Sequence Similarity 5, Member C (FAM5C) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
Gene Symbol | BRINP3 |
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Gene Full Name | BMP/retinoic acid inducible neural specific 3 |
Alias Symbols | BMP/retinoic acid-inducible neural-specific protein 3;bone morphogenetic protein/retinoic acid inducible neural-specific 3;DBCCR1L;DBCCR1L1;DBCCR1-like protein 1;FAM5C;family with sequence similarity 5, member C;protein FAM5C. |
NCBI Gene Id | 339479 |
Protein Name | BMP/retinoic acid-inducible neural-specific protein 3 |
Description of Target | Inhibits neuronal cell proliferation by negative regulation of the cell cycle transition. Promotes pituitary gonadotrope cell proliferation, migration and invasion, when overexpressed. May play a role in cell pituitary tumor development. |
Uniprot ID | Q76B58 |
Protein Accession # | NP_001304117 |
Nucleotide Accession # | NM_001317188.1 |
Protein Size (# AA) | 766 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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