BRD4 Antibody - N-terminal region (OAAB20950)

Catalog No: OAAB20950

Size:400ul

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Clone: RB3419
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: IHC-P
• Additional Information: Function: BRD4 is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. The gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This BRD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human BRD4.
• Purification: SAS precipitation, followed by dialysis against PBS
• Predicted Homology Based on Immunogen Sequence: Human, Mouse
• Concentration: 2 mg/mL
• Preservative: 0.09% Sodium Azide
• Formulation: Purified polyclonal antibody, supplied in PBS with 0.09% sodium azide.
• Application Info: IHC-P: 1:50~100
• Reference: Gagnon, D. et al. J Virol. May; 83(9): 4127?139(2009).
  Maruyama, T., et al., Mol. Cell. Biol. 22(18):6509-6520 (2002).
  French, C.A., et al., Am. J. Pathol. 159(6):1987-1992 (2001).
  Dey, A., et al., Mol. Cell. Biol. 20(17):6537-6549 (2000).

Target Info

• Gene Symbol: BRD4
• Gene Full Name: bromodomain containing 4
• Alias Symbols: CAP, MCAP, HUNK1, HUNKI
• NCBI Gene Id: 23476
• Description of Target: The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.
• Uniprot ID: O60885
• Protein Accession #: NP_055114.1
• Nucleotide Accession #: NM_014299.2
• Molecular Weight: 152 kDa