Catalog No: OKCD04290
Size:96WELLS
Price: $700.00
SKU
OKCD04290
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Datasheets/ManualsPrintable datasheet for BRAK ELISA Kit (Rat) (OKCD04290)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)92-101%89-96%86-94%86-102%
EDTA Plasma (n=5)82-103%85-97%88-99%96-105%
Heparin Plasma (n=5)78-97%82-103%88-102%80-98%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine (C-X-C Motif) Ligand 14 (CXCL14). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine (C-X-C Motif) Ligand 14 (CXCL14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine (C-X-C Motif) Ligand 14 (CXCL14), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Chemokine (C-X-C Motif) Ligand 14 (CXCL14) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average (%)
Serum (n=5)95-104101
EDTA Plasma (n=5)86-10599
Heparin Plasma (n=5)85-9590
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine (C-X-C Motif) Ligand 14 (CXCL14) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine (C-X-C Motif) Ligand 14 (CXCL14) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Brak Microplate96 Wells (12 x 8 Well strips)
Brak Lyophilized Standard2
100X Brak HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity6.2 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCxcl14
Gene Full NameC-X-C motif chemokine ligand 14
Alias SymbolsBRAK;chemokine (C-X-C motif) ligand 14;C-X-C motif chemokine 14.
NCBI Gene Id306748
Protein NameBRAK
Uniprot IDD4A5S7
Protein Accession #NP_001013155.1
Nucleotide Accession #NM_001013137.2
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