BRAF ELISA Kit (Human) (OKCA02146)

Catalog No: OKCA02146

Size:96 Wells

Human BRAF ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Sample Type	Dilution	Mean (%)	Range (%)
  Serum (n=4)	Neat	90	80-100
  Serum (n=4)	1:2	97	91-105
  Serum (n=4)	1:4	99	92-110
  Serum (n=4)	1:8	92	86-98

• ELISA Kit Principle: Aviva Systems Biology BRAF ELISA Kit (Human) (OKCA02146) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BRAF has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BRAF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample BRAF captured in well.
• ELISA Kit Range: 31.25 pg/mL-2,000 pg/mL
• ELISA Kit Recovery:

  Sample Type	Mean (%)	Range (%)
  Serum (n=5)	95	89-104
  EDTA plasma (n=4)	97	90-100

• ELISA Kit Reproducibility: Mean Intra-Assay Variation <10% CV (n=20)
  Mean Inter-Assay Variation <12% CV (n=8)
• ELISA Kit Component:
  • Anti-BRAF Microplate: 96 Wells (12 x 8 Well strips)
  • BRAF Lyophilized Standard: 2 x 2 ng
  • 100X Biotinylated BRAF Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Lysates
• Sensitivity: 7.8 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: BRAF
• Alias Symbols: NS7, B-raf, BRAF1, RAFB1, B-RAF1
• NCBI Gene Id: 673
• Protein Name: Serine/threonine-protein kinase B-raf
• Description of Target: Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
• Uniprot ID: P15056
• Protein Accession #: NP_004324
• Nucleotide Accession #: NM_004333