BPI ELISA Kit (Mouse) (OKCD02406)

Catalog No: OKCD02406

Size:96WELLS

Mouse BPI ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	79-92%	84-93%	94-103%	78-105%
  EDTA Plasma (n=5)	92-99%	89-103%	78-104%	82-101%
  Heparin Plasma (n=5)	92-105%	81-90%	92-101%	93-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bactericidal/Permeability Increasing Protein (BPI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bactericidal/Permeability Increasing Protein (BPI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bactericidal/Permeability Increasing Protein (BPI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Bactericidal/Permeability Increasing Protein (BPI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 31.2-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	93-101	96
  EDTA Plasma (n=5)	93-105	97
  Heparin Plasma(n=5)	83-97	87

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bactericidal/Permeability Increasing Protein (BPI) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bactericidal/Permeability Increasing Protein (BPI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-BPI Microplate: 96 Wells (12 x 8 Well strips)
  • BPI Lyophilized Standard: 2
  • 100X Biotinylated BPI Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 13.7 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bactericidal/Permeability Increasing Protein (BPI). No significant cross-reactivity or interference between Bactericidal/Permeability Increasing Protein (BPI) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Bpi
• Gene Full Name: bactericidal permeablility increasing protein
• Alias Symbols: 9230105K17Rik;bactericidal permeability-increasing protein;Bpif;Bpifd1.
• NCBI Gene Id: 329547
• Protein Name: Bactericidal permeability-increasing protein
• Description of Target: The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope.
• Uniprot ID: Q67E05
• Protein Accession #: NP_808518.1
• Nucleotide Accession #: NM_177850.3
• Protein Size (# AA): 483