BPI ELISA Kit (Human) (OKCD07235)

Catalog No: OKCD07235

Size:96WELLS

BPI ELISA Kit (Human) (OKCD07235) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bactericidal/Permeability Increasing Protein (BPI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bactericidal/Permeability Increasing Protein (BPI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bactericidal/Permeability Increasing Protein (BPI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Bactericidal/Permeability Increasing Protein (BPI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 12.5-800pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bactericidal/Permeability Increasing Protein (BPI) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bactericidal/Permeability Increasing Protein (BPI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • BPI Microplate: 96 Wells (12 x 8 Well strips)
  • BPI Lyophilized Standard: 2
  • 100X BPI HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 5.3pg/mL

Target Info

• Gene Symbol: BPI
• Gene Full Name: bactericidal permeability increasing protein
• Alias Symbols: bactericidal permeability-increasing protein;BPI fold containing family D, member 1;BPIFD1;CAP 57;rBPI;recombinant BPI holoprotein, rBPI.
• NCBI Gene Id: 671
• Protein Name: Bactericidal permeability-increasing protein
• Description of Target: The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa.
• Uniprot ID: P17213
• Protein Accession #: NP_001716.2
• Nucleotide Accession #: NM_001725.2