Rabbit Anti-Bovine IgG [H&L] Polyclonal Antibody - PE Conjugated (OARA04558)

Catalog No: OARA04558

Size:500UG

Product Info

• Predicted Species Reactivity: Bovine
• Product Format: Lyophilized
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: R-PE
• Application: Flow cytometry|Immunofluorescence
• Additional Information: Color: Red
  Conjugation Reference: Modified from the method of Carlsson J, Drevin H, and Axen R. Protein thiolation and reversibleprotein-protein conjugation. Biochem J 173, 723-737 (1978).
  Emission Wavelength: 575
  Excitation Wavelength: 488
• Reconstitution and Storage: Store vial at 4C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
• Immunogen: Bovine IgG whole molecule
• Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
• Concentration: 1 mg/ml
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Bovine IgG and Bovine Serum. No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).
• Application Info: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
  iF: 1:100 - 1:250
  FLOW CYTOMETRY 1:100 - 1:250