Catalog No: OKWB00104
Size:96T
Price: $450.00
SKU
OKWB00104
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for BOLA-DQA1 ELISA Kit (Bovine) (OKWB00104) |
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Predicted Species Reactivity | Bos taurus|Bovine | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | Aviva Systems Biology Bola-dqa1 ELISA Kit (Bovine) (OKWB00104) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bola-dqa1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Bola-dqa1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Bola-dqa1 captured in the well. | ||||||||||||||||||||||
ELISA Kit Range | 1.563-100ng/ml | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Mean Intra-Assay Precision <10% Mean Inter-Assay Precision <12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids | ||||||||||||||||||||||
Sensitivity | 0.938 ng/mL | ||||||||||||||||||||||
Assay Info | Assay Type: Quantitative Sandwich ELISA |
Gene Symbol | LOC100848815 |
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Gene Full Name | SLA class II histocompatibility antigen, DQ haplotype D alpha chain-like |
Alias Symbols | BoLA-DQA;BoLA-DQA1;DQA;MHC class II antigen;MHC LA DQA alpha-5;MHC lymphocyte antigen;SLA class II histocompatibility antigen, DQ haplotype D alpha chain-like. |
NCBI Gene Id | 100848815 |
Protein Name | BOLA-DQA1 protein |
Uniprot ID | A6H6Y1 |
Protein Accession # | NP_001306818 |
Nucleotide Accession # | NM_001319889 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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