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Catalog No: OKWB00104
Size:96T
Price: $450.00
SKU
OKWB00104
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Datasheets/ManualsPrintable datasheet for BOLA-DQA1 ELISA Kit (Bovine) (OKWB00104)
Product Info
Predicted Species ReactivityBos taurus|Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit Linearity
Sample Type1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)88-104%86-105%85-105%89-105%
EDTA Plasma (n=5)91-100%87-101%83-100%92-100%
Heparin Plasma (n=5)86-100%80-98%82-93%83-100%
ELISA Kit PrincipleAviva Systems Biology Bola-dqa1 ELISA Kit (Bovine) (OKWB00104) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bola-dqa1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Bola-dqa1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Bola-dqa1 captured in the well.
ELISA Kit Range1.563-100ng/ml
ELISA Kit Recovery
Sample TypeRangeMean
Serum (n=5)92-10598
EDTA Plasma (n=5)91-10598
Heparin Plasma (n=5)86-9693
ELISA Kit ReproducibilityMean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%
ELISA Kit Component
ComponentAmount
Bola-dqa1 Microplate96 Wells (12 x 8 Well strips)
Bola-dqa1 Lyophilized Standard2 x 100 ng
100X Bola-dqa1 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Sensitivity0.938 ng/mL
Assay InfoAssay Type: Quantitative Sandwich ELISA
Gene SymbolLOC100848815
Gene Full NameSLA class II histocompatibility antigen, DQ haplotype D alpha chain-like
Alias SymbolsBoLA-DQA;BoLA-DQA1;DQA;MHC class II antigen;MHC LA DQA alpha-5;MHC lymphocyte antigen;SLA class II histocompatibility antigen, DQ haplotype D alpha chain-like.
NCBI Gene Id100848815
Protein NameBOLA-DQA1 protein
Uniprot IDA6H6Y1
Protein Accession #NP_001306818
Nucleotide Accession #NM_001319889
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