BNP ELISA Kit (Pig) (OKCD02405)

Catalog No: OKCD02405

Size:96WELLS

Pig BNP ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Porcine|Sus scrofa
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	98-105%	78-89%	85-92%	84-99%
  EDTA Plasma (n=5)	96-103%	79-93%	89-102%	96-104%
  Heparin Plasma (n=5)	96-105%	80-88%	88-95%	88-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Brain Natriuretic Peptide (BNP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Brain Natriuretic Peptide (BNP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Brain Natriuretic Peptide (BNP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Brain Natriuretic Peptide (BNP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 31.2-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	95-102	99
  EDTA Plasma (n=5)	86-101	96
  Heparin Plasma(n=5)	89-101	97

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Brain Natriuretic Peptide (BNP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Brain Natriuretic Peptide (BNP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-BNP Microplate: 96 Wells (12 x 8 Well strips)
  • BNP Lyophilized Standard: 2
  • 100X Biotinylated BNP Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 11.7 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Brain Natriuretic Peptide (BNP). No significant cross-reactivity or interference between Brain Natriuretic Peptide (BNP) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: NPPB
• Gene Full Name: natriuretic peptide B
• Alias Symbols: BNP;BNP1;brain natriuretic factor prohormone;brain natriuretic peptide;gamma-brain natriuretic peptide;Iso-ANP;natriuretic peptides B;preproBNP;proBNP.
• NCBI Gene Id: 396844
• Protein Name: Natriuretic peptides B
• Description of Target: Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3 (By similarity).
• Uniprot ID: P07634
• Protein Accession #: NP_999011.1
• Nucleotide Accession #: NM_213846.1
• Protein Size (# AA): 131