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Datasheets/Manuals | Printable datasheet for BNIP3L Antibody (OAPB00112) (OAPB00112) |
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Predicted Species Reactivity | Bovine|Human|Mouse |
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Product Format | Liquid. Bnip3L Antibody is supplied in PBS containing 0.02% sodium azide. |
Clonality | Polyclonal |
Host | Rabbit |
Conjugation | Unconjugated |
Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot |
:: | Buffer: Antibody is supplied in PBS containing 0.02% sodium azide. |
Reconstitution and Storage | 2°C to 8°C |
Immunogen | Bnip3L antibody was raised against a 16 amino acid peptide near the center of human Bnip3L. The immunogen is located within amino acids 60 - 110 of Bnip3L. |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Human |
Concentration | 1 mg/ml |
Blocking Peptide | For anti-BNIP3L (OAPB00112) antibody is Catalog # OEPB02232 |
Application Info | Bnip3L antibody can be used for detection of Bnip3L by Western blot at 0.5 to 1 ug/ml. The approximate 40 kDa top band represents monomer of Bnip3L (4). It is human and mouse reactive. (Optimal dilution should be determined by user) |
Reference | Matsushima M, Fujiwara T, Takahashi E, et al. Isolation, mapping, and functional analysis of a novel human cDNA (BNIP3L) encoding a protein homologous to human NIP3. Genes Chromosomes Cancer 1998;21:230-5 ;Yasuda M, Han JW, Dionne CA, Boyd JM, Chinnadurai G. BNIP3alpha: a human homolog of mitochondrial proapoptotic protein BNIP3. Cancer Res 1999;59:533-7 ;Chen G, Cizeau J, Vande Velde C, et al. Nix and Nip3 form a subfamily of pro-apoptotic mitochondrial proteins. J Biol Chem 1999;274(1):7-10 ;Imazu T, Shimizu S, Tagami S, et al. Bcl-2/E1B 19 kDa-interacting protein 3-like protein (Bnip3L) interacts with bcl-2/Bcl-xL and induces apoptosis by altering mitochondrial membrane permeability. Oncogene. 1999;18:4523-9 (RD1200) |
Gene Symbol | BNIP3L |
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Gene Full Name | BCL2 interacting protein 3 like |
Alias Symbols | adenovirus E1B19k-binding protein B5;BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A;BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like;BCL2/adenovirus E1B 19-kd protein-interacting protein 3a;BCL2/adenovirus E1B 19kDa interacting protein 3 like;BNIP3a;NIP3L;NIP3-like protein X;NIP-3-like protein X;NIX. |
NCBI Gene Id | 665 |
Protein Name | BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like|BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like isoform 1 [Homo sapiens] |
Description of Target | Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. Inhibits apoptosis induced by BNIP3. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. May function as a tumor suppressor. |
Uniprot ID | O60238 |
Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/NP_004322 |
Nucleotide Accession # | NM_004331.2; XM_005273617.2 |
Molecular Weight | Predicted: 24 kDa Observed: 36, 40 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "BNIP3L Antibody (OAPB00112)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Bovine|Human|Mouse".
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How long will it take to receive "BNIP3L Antibody (OAPB00112)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "BNIP3L Antibody (OAPB00112)" provided in?
This item is provided in "Liquid. Bnip3L Antibody is supplied in PBS containing 0.02% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "BNIP3L Antibody (OAPB00112)"?
This target may also be called "adenovirus E1B19k-binding protein B5;BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A;BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like;BCL2/adenovirus E1B 19-kd protein-interacting protein 3a;BCL2/adenovirus E1B 19kDa interacting protein 3 like;BNIP3a;NIP3L;NIP3-like protein X;NIP-3-like protein X;NIX." in publications.
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What is the shipping cost for "BNIP3L Antibody (OAPB00112)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "BNIP3L Antibody (OAPB00112)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "BNIP3L Antibody (OAPB00112)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "Predicted: 24 kDa Observed: 36, 40 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "BNIP3L Antibody (OAPB00112)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "BNIP3L"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "BNIP3L"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "BNIP3L"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "BNIP3L"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "BNIP3L"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "BNIP3L"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.