BNIP3L Antibody (OAGA03904)

Catalog No: OAGA03904

Size:100 ul

Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with BNIP3L antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Sample (30 ug of whole cell lysate)
A: Molt-4
12% SDS PAGE
diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using BNIP3L antibody at 1:500 dilution.

Immunofluorescence analysis of methanol-fixed HeLa, using BNIP3L antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Bovine
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human BNIP3L. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.44 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: BNIP3L
• Gene Full Name: BCL2 interacting protein 3 like
• Alias Symbols: NIX, BNIP3a
• NCBI Gene Id: 665
• Protein Name: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like
• Description of Target: This gene encodes a protein that belongs to the pro-apoptotic subfamily within the Bcl-2 family of proteins. The encoded protein binds to Bcl-2 and possesses the BH3 domain. The protein directly targets mitochondria and causes apoptotic changes, including loss of membrane potential and the release of cytochrome c.
• Uniprot ID: O60238
• Protein Accession #: NP_001317420.1
• Nucleotide Accession #: NM_001330491.1