BNIP3 ELISA Kit (Mouse) (OKEH08196)

Catalog No: OKEH08196

Size:96T

Mouse BNIP3 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	92-101% (n = 20)	105-117% (n = 20)	105-115% (n = 20)	88-98% (n = 20)
  EDTA plasma(n=5)	108-108% (n = 20)	91-91% (n = 20)	98-106% (n = 20)	82-92% (n = 20)
  heparin plasma(n=5)	113-122% (n = 20)	85-93% (n = 20)	87-99% (n = 20)	108-117% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology BNIP3 ELISA Kit (Mouse) (OKEH08196) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BNIP3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BNIP3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BNIP3 captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 80% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=3.9% (n = 20)
  Mean Inter-assay CV%: <=5.4% (n = 20)
• ELISA Kit Component:
  • BNIP3 Microplate: 96 Wells (12 x 8 Well strips)
  • BNIP3 Lyophilized Standard: 2
  • 100X Biotinylated BNIP3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2 (By similarity). Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane may play a critical role in the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix (By similarity). The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix (By similarity). Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway.
  Subcellular Location: Mitochondrion Mitochondrion outer membrane Single-pass membrane protein Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria. Colocalizes with SPATA18 at the mitochondrion outer membrane (By similarity).
  Unigene: Mm.378890
  SMR: O55003
  String: 10090.ENSMUSP00000101718
  KEGG: mmu:12176
  Pfam: PF06553
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 19pg/mL
• Specificity: Natural and recombinant Mouse BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Bnip3
• Gene Full Name: BCL2/adenovirus E1B interacting protein 3
• Alias Symbols: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3;BCL2/adenovirus E1B 19 kDa-interacting protein 1, NIP3;BCL2/adenovirus E1B 19kDa-interacting protein 1, NIP3;BCL2/adenovirus E1B interacting protein 1, NIP3;N;Nip3.
• NCBI Gene Id: 12176
• Protein Name: O55003
• Description of Target: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
• Uniprot ID: https://www.uniprot.org/uniprot/O55003
• Protein Accession #: NP_033890.1
• Nucleotide Accession #: NM_009760.4