BNIP3 Chemi-Luminescent ELISA Kit (Rat) (OKCD03336)

Catalog No: OKCD03336

Size:96WELLS

Rat BNIP3 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) level in the sample or standard.
• ELISA Kit Range: 54.9-40,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Bnip3 Microplate: 96 Wells (12 x 8 Well strips)
  • Bnip3 Lyophilized Standard: 2
  • 100X Bnip3 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.625 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Bnip3
• Gene Full Name: BCL2 interacting protein 3
• Alias Symbols: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3;BCL2/adenovirus E1B 19 kDa-interacting protein 3;BCL2/adenovirus E1B interacting protein 3.
• NCBI Gene Id: 84480
• Protein Name: BCL2-interacting protein 3
• Description of Target: BCL2-interacting protein 3
• Uniprot ID: Q9ET45
• Protein Accession #: NP_445872
• Nucleotide Accession #: NM_053420