BMX ELISA Kit (Mouse) (OKCD02404)

Catalog No: OKCD02404

Size:96WELLS

Mouse BMX ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to BMX Non Receptor Tyrosine Kinase (BMX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to BMX Non Receptor Tyrosine Kinase (BMX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BMX Non Receptor Tyrosine Kinase (BMX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of BMX Non Receptor Tyrosine Kinase (BMX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BMX Non Receptor Tyrosine Kinase (BMX) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BMX Non Receptor Tyrosine Kinase (BMX) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-BMX Microplate: 96 Wells (12 x 8 Well strips)
  • BMX Lyophilized Standard: 2
  • 100X Biotinylated BMX Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.113 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of BMX Non Receptor Tyrosine Kinase (BMX). No significant cross-reactivity or interference between BMX Non Receptor Tyrosine Kinase (BMX) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Bmx
• Gene Full Name: BMX non-receptor tyrosine kinase
• Alias Symbols: bone marrow tyrosine kinase gene in chromosome X protein homolog;cytoplasmic tyrosine-protein kinase BMX;Etk;Etk/Bmx;Tyr;Tyro8;TYRO8 protein tyrosine kinase 8.
• NCBI Gene Id: 12169
• Protein Name: Cytoplasmic tyrosine-protein kinase BMX
• Description of Target: Non-receptor tyrosine kinase that plays central but diverse modulatory roles in various signaling processes involved in the regulation of actin reorganization, cell migration, cell proliferation and survival, cell adhesion, and apoptosis. Participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein coupled receptors, antigen receptors and integrins. Induces tyrosine phosphorylation of BCAR1 in response to integrin regulation. Activation of BMX by integrins is mediated by PTK2/FAK1, a key mediator of integrin signaling events leading to the regulation of actin cytoskeleton and cell motility. Plays a critical role in TNF-induced angiogenesis, and implicated in the signaling of TEK and FLT1 receptors, 2 important receptor families essential for angiogenesis. Required for the phosphorylation and activation of STAT3, a transcription factor involved in cell differentiation. Also involved in interleukin-6 (IL6) induced differentiation. Plays also a role in programming adaptive cytoprotection against extracellular stress in different cell systems, salivary epithelial cells, brain endothelial cells, and dermal fibroblasts. May be involved in regulation of endocytosis through its interaction with an endosomal protein RUFY1. May also play a role in the growth and differentiation of hematopoietic cells; as well as in signal transduction in endocardial and arterial endothelial cells.
• Uniprot ID: P97504
• Protein Accession #: NP_033889.2
• Nucleotide Accession #: NM_009759.4
• Protein Size (# AA): 651