BMPR2 ELISA Kit (Mouse) (OKCA02346)

Catalog No: OKCA02346

Size:96 Wells

Mouse BMPR2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	105	85-110
  1:2	101	93-104
  1:4	96	88-100
  1:8	100	92-104

• ELISA Kit Principle: Aviva Systems Biology Bmpr2 ELISA Kit (Mouse) (OKCA02346) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bmpr2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Bmpr2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Bmpr2 captured in the well.
• ELISA Kit Range: 62.5 - 4,000 pg/mL
• ELISA Kit Recovery:

  Sample Type	Mean Recovery (%)	Range (%)
  Serum (n=5)	110	98-114
  EDTA Plasma (n=4)	100	89-105

• ELISA Kit Reproducibility: Mean Intra-Assay: CV <12% (n=20)
  Mean Inter-Assay: CV <15% (n=24)
• ELISA Kit Component:
  • Bmpr2 Microplate: 96 Wells (12 x 8 Well strips)
  • Bmpr2 Lyophilized Standard: 2
  • 100X Biotinylated Bmpr2 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates And Cell Lysates
• Sensitivity: 15.6 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: BMPR2
• Alias Symbols: BM, BMP, BMP-, BMP-2, BRK-3, BMPR-2, BMPRII, BMPR-II, Gm20272, AL117858, AW546137, BB189135, 2610024H22Rik
• NCBI Gene Id: 12168
• Protein Name: Bone morphogenetic protein receptor type-2
• Description of Target: On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Binds to BMP7, BMP2 and, less efficiently, BMP4. Binding is weak but enhanced by the presence of type I receptors for BMPs. Mediates induction of adipogenesis by GDF6 .
• Uniprot ID: O35607
• Protein Accession #: NP_031587
• Nucleotide Accession #: NM_007561