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Catalog No: OKCD00783 (Formerly GWB-KBBUQ1)
Size:96WELLS
Price: $650.00
SKU
OKCD00783
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Datasheets/ManualsPrintable datasheet for BMPR2 ELISA Kit (Human) (OKCD00783)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
83-104%83-94%80-92%86-95%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein Receptor 2 (BMPR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein Receptor 2 (BMPR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein Receptor 2 (BMPR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Bone Morphogenetic Protein Receptor 2 (BMPR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
94-10298
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein Receptor 2 (BMPR2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein Receptor 2 (BMPR2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-BMPR2 Microplate96 Wells (12 x 8 Well Strips)
BMPR2 Lyophilized Standard2 x 20 ng
100X Biotinylated BMPR2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids.
Sensitivity< 0.124 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein Receptor 2 (BMPR2). No significant cross-reactivity or interference between Bone Morphogenetic Protein Receptor 2 (BMPR2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolBMPR2
Gene Full Namebone morphogenetic protein receptor type 2
Alias SymbolsBMP type II receptor;BMP type-2 receptor;BMPR3;BMPR-II;BMR2;bone morphogenetic protein receptor type II;bone morphogenetic protein receptor type-2;bone morphogenetic protein receptor, type II (serine/threonine kinase);BRK-3;POVD1;PPH1;T-ALK;type II activin receptor-like kinase;type II receptor for bone morphogenetic protein-4.
NCBI Gene Id659
Protein NameBone morphogenetic protein receptor type-2
Description of TargetOn ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Binds to BMP7, BMP2 and, less efficiently, BMP4. Binding is weak but enhanced by the presence of type I receptors for BMPs. Mediates induction of adipogenesis by GDF6.
Uniprot IDQ13873
Protein Accession #NP_001195
Nucleotide Accession #NM_001204.6
Protein Size (# AA)1038
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