BMPR1B ELISA Kit (Human) (OKCD01535)

Catalog No: OKCD01535 (Formerly GWB-KBBXJ4)

Size:96WELLS

Human BMPR1B ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein Receptor 1B (BMPR1B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein Receptor 1B (BMPR1B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein Receptor 1B (BMPR1B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Bone Morphogenetic Protein Receptor 1B (BMPR1B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein Receptor 1B (BMPR1B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein Receptor 1B (BMPR1B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-BMPR1B Microplate: 96 Wells (12 x 8 Well Strips)
  • BMPR1B Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated BMPR1B Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.117 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein Receptor 1B (BMPR1B). No significant cross-reactivity or interference between Bone Morphogenetic Protein Receptor 1B (BMPR1B) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: BMPR1B
• Gene Full Name: bone morphogenetic protein receptor type 1B
• Alias Symbols: activin receptor-like kinase 6;ALK6;ALK-6;AMDD;BDA1D;BDA2;BMP type-1B receptor;BMPR-1B;bone morphogenetic protein receptor type-1B;bone morphogenetic protein receptor, type IB;CDw293;serine/threonine receptor kinase.
• NCBI Gene Id: 658
• Protein Name: Bone morphogenetic protein receptor type-1B
• Description of Target: On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for BMP7/OP-1 and GDF5. Positively regulates chondrocyte differentiation through GDF5 interaction.
• Uniprot ID: O00238
• Protein Accession #: NP_001194.1
• Nucleotide Accession #: NM_001203.2
• Protein Size (# AA): 502