Catalog No: OKCD04448
Size:96WELLS
Price: $650.00
SKU
OKCD04448
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Datasheets/ManualsPrintable datasheet for BMP7 ELISA Kit (Rat) (OKCD04448)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)98-105%94-105%96-103%85-97%
EDTA Plasma (n=5)78-96%90-98%95-103%80-101%
Heparin Plasma (n=5)86-104%95-104%87-98%97-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 7 (BMP7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 7 (BMP7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein 7 (BMP7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Bone Morphogenetic Protein 7 (BMP7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average (%)
Serum (n=5)81-10296
EDTA Plasma (n=5)79-9790
Heparin Plasma (n=5)95-10298
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 7 (BMP7) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 7 (BMP7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Alk-Smase Microplate96 Wells (12 x 8 Well strips)
Alk-Smase Lyophilized Standard2
100X Alk-Smase HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity5.9 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolBmp7
Gene Full Namebone morphogenetic protein 7
Alias SymbolsBMP-7;bone moorphogenic protein-7;bone morphogenetic protein 7.
NCBI Gene Id85272
Protein NameBone morphogenetic protein 7
Description of Targetbinds to cell surface heparan sulfate [RGD, Feb 2006]
Uniprot IDG3V6W8
Protein Accession #NP_001178785.1
Nucleotide Accession #NM_001191856.2
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