Size:96 Wells
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BMP7 ELISA Kit (Porcine) (OKEI00639)

Catalog#: OKEI00639
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species Reactivity Porcine
Kit Detection Method Colorimetric, OD450 nm
Kit Duration ~ 3 Hours
Kit Principle Aviva Systems Biology Bmp7 ELISA Kit (Porcine) (OKEI00639) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bmp7 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Bmp7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample Bmp7
Kit Range 0.031 - 2 ng/mL
Kit Reproducibility Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level Bmp7. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level Bmp7 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
Kit Component
Bmp7 Microplate96 Wells (12 x 8 Well strips)
Bmp7 Lyophilized Standard2
100X Bmp7 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 10 mL
30X Wash Buffer1 x 30 mL
100X Luminol Substrate1 x 10 mL
Substrate Diluent1 x 10 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, Plasma, Biological Fluids
Sensitivity 18.75 pg/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol BMP7
NCBI Gene Id 492315
Protein Name Bone morphogenetic protein 7 (Osteogenic protein 1)
Swissprot Id A5GFN1
Protein Accession # NP_001098760
Nucleotide Accession # NM_001105290
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express BMP7.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express BMP7.
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