BMP7 ELISA Kit (Mouse) (OKBB00993)

Catalog No: OKBB00993

Size:96 wells

BMP7 ELISA Kit (Mouse) (OKBB00993) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's mouse BMP-7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for BMP-7 has been precoated onto 96-well plates. Standards(Expression system for standard: CHO, Immunogen sequence: S292-H430) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse BMP-7 amount of sample captured in plate.
• ELISA Kit Range: 31.2 pg/ml - 2,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	82	219	964	87	198	895
  Standard deviation	4.83	14.45	74.22	6.78	13.46	84.13
  CV(%)	5.9	6.6	7.7	7.8	6.8	9.4

• ELISA Kit Component:
  • 96-Well Plate Pre-coated with Anti-Mouse BMP7 Antibody: 96 Wells (12 x 8 Well Strips)
  • Lyophilized Recombinant Mouse BMP7 Standard: 2 x 10 ng
  • 100X Biotinylated Anti-Mouse BMP7 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Additional Information:

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	82	84	93	90	87	4.43	5%
  Sample 2	219	249	237	227	233	11.22	4.8%
  Sample 3	964	880	981	967	948	39.78	4.1%

  *number of samples for each test n=16.

  
  

  Standard Number	8	7	6	5	4	3	2	1
  Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
  O.D.	0.032	0.085	0.143	0.248	0.423	0.816	1.359	2.231

• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: bone tissue,cell culture supernates, serum and plasma ((heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant mouse BMP-7
• Immunogen: Expression system for standard: CHO; Immunogen sequence: S292-H430
• Description: For quantitative detection of mouse BMP-7 in bone tissue, cell culture supernatants, serum and plasma((heparin, EDTA).

Target Info

• Gene Symbol: BMP7
• Gene Full Name: bone morphogenetic protein 7
• Alias Symbols: BMP 7, BMP7, BMP-7, Bone morphogenetic protein 7, Eptotermin alfa, OP 1, OP1, OP1-, Osteogenic protein 1
• NCBI Gene Id: 12162
• Protein Name: Bone morphogenetic protein 7
• Description of Target: Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-β superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.
• Uniprot ID: P23359
• Protein Accession #: NP_031583.2
• Nucleotide Accession #: NM_007557.3