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BMP7 ELISA Kit (Human) (OKCD06834)

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Predicted Species Reactivity:
Human
Application:
ELISA
Reconstitution and Storage:
Store as indicated in product manual.
Gene Symbol:
BMP7
Official Gene Full Name:
bone morphogenetic protein 7
Alias Symbols:
OP1; Osteogenic Protein-1; Eptotermin alfa
NCBI Gene Id:
655
Protein Name:
Bone morphogenetic protein 7
Description of Target:
The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP has a proposed role in early development. In addition, the fact that this BMP is closely related to BMP5 and BMP7 has lead to speculation of possible bone inductive activity.The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development. In addition, the fact that this BMP is closely related to BMP5 and BMP7 has lead to speculation of possible bone inductive activity.
Swissprot Id:
P18075
Protein Accession #:
NP_001710.1
Nucleotide Accession #:
NM_001719.2
Sample Type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
< 27.4pg/mL
Kit Range:
62.5-4000pg/mL
Kit Reproducibility:
Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level BMP7. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level BMP7 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology BMP7 ELISA Kit (Human) (OKCD06834) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BMP7 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BMP7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BMP7 captured in the well.
Kit Component:
ComponentAmount
BMP7 Microplate96 Wells (12 x 8 Well strips)
BMP7 Lyophilized Standard2
100X BMP7 HRP-Detector Anitbody1 x 120 µL
100X Avidin-HRP Conjugate1 x 120 µL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by RNA-seq analysis to express BMP7.
RNA Seq:
Find tissues and cell lines supported by DNA array analysis to express BMP7.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.

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