Catalog No: OKBB00994
Size:96 wells
Price: $538.00
SKU
OKBB00994
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHamster
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva's Hamster BMP-7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for BMP-7 has been precoated onto 96-well plates. Standards(Expression system for standard: CHO, Immunogen sequence: S292-H430) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the Hamster BMP-7 amount of sample captured in plate.
ELISA Kit Range31.2 pg/ml - 2,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)18566514082108871699
Standard deviation9.2543.8966.1814.9170.96132.52
CV(%)5.06.64.77.18.07.8
ELISA Kit Component
ComponentAmount
96-Well Plate Pre-coated with Anti-Hamster Bmp7 Antibody96 Wells (12 x 8 Well Strips)
Lyophilized Recombinant Hamster Bmp7 Standard2 x 10 ng
100X Biotinylated Anti-Hamster Bmp7 Antibody1 x 130 uL
100X Avidin-Biotin Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Additional Information

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

LotsLot1 (pg/ml)Lot2 (pg/ml)Lot3 (pg/ml)Lot4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 182849390874.435%
Sample 221924923722723311.224.8%
Sample 396488098196794839.784.1%

*number of samples for each test n=16.

::
Standard Number87654321
Concentration (pg/ml)02000200020002000200020002000
O.D.0.0320.1430.1430.2480.4230.8161.3592.231
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeSandwich High Sensitivity ELISA kit for Quantitative Detection of Chinese hamster BMP-7
Sensitivity<10 pg/ml
ImmunogenExpression system for standard: CHO; Immunogen sequence: S292-H430
DescriptionSandwich high sensitivity ELISA kit for Quantitative Detection of Chinese hamster BMP-7. 96wells/kit, with removable strips.
Gene SymbolBMP7
Gene Full NameBone morphogenetic protein 7
Alias SymbolsBone morphogenetic protein 7;BMP-7;Osteogenic protein 1;OP-1;Bmp7;Bmp-7, Op1;
NCBI Gene Id100759897
Protein NameBone morphogenetic protein 7
Description of TargetBone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-β superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.
Uniprot IDP23359
Protein Accession #NP_031583.2
Nucleotide Accession #NM_007557.3
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