Catalog No: OKCD02402
Size:96WELLS
Price: $625.00
SKU
OKCD02402
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Datasheets/ManualsPrintable datasheet for BMP6 ELISA Kit (Mouse) (OKCD02402)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)83-94%82-101%84-101%96-104%
EDTA Plasma (n=5)78-95%88-99%78-97%93-103%
Heparin Plasma (n=5)81-93%87-98%96-104%98-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 6 (BMP6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 6 (BMP6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein 6 (BMP6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Bone Morphogenetic Protein 6 (BMP6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range31.2-2,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)81-9188
EDTA Plasma (n=5)90-9794
Heparin Plasma(n=5)84-9794
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 6 (BMP6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 6 (BMP6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-BMP6 Microplate96 Wells (12 x 8 Well strips)
BMP6 Lyophilized Standard2
100X Biotinylated BMP6 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity6.0 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 6 (BMP6). No significant cross-reactivity or interference between Bone Morphogenetic Protein 6 (BMP6) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolBmp6
Gene Full Namebone morphogenetic protein 6
Alias Symbolsbone morphogenetic protein 6;D13Wsu115;D13Wsu115e;Vg;VG-1-related protein;Vgr1.
NCBI Gene Id12161
Protein NameBone morphogenetic protein 6
Description of TargetInduces cartilage and bone formation.
Uniprot IDP20722
Protein Accession #NP_031582.1
Nucleotide Accession #NM_007556.3
Protein Size (# AA)510
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