BMP6 ELISA Kit (Mouse) (OKCA02160)

Catalog No: OKCA02160

Size:96 Wells

Mouse BMP6 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Sample Type	Dilution	Mean (%)	Range (%)
  Serum (n=4)	Neat	96	90-102
  Serum (n=4)	1:2	96	82-101
  Serum (n=4)	1:4	98	93-107
  Serum (n=4)	1:8	97	92-105

• ELISA Kit Principle: Aviva Systems Biology Bmp6 ELISA Kit (Mouse) (OKCA02160) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BMP6 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BMP6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample BMP6 captured in well.
• ELISA Kit Range: 31.25 pg/mL-2,000 pg/mL
• ELISA Kit Recovery:

  Sample Type	Mean (%)	Range (%)
  Serum (n=5)	100	95-105
  EDTA plasma (n=4)	102	96-108

• ELISA Kit Reproducibility: Mean Intra-Assay Variation <10% CV (n=20)
  Mean Inter-Assay Variation <12% CV (n=8)
• ELISA Kit Component:
  • Anti-BMP6 Microplate: 96 Wells (12 x 8 Well strips)
  • BMP6 Lyophilized Standard: 2 x 2 ng
  • 100X Biotinylated BMP6 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Culture Supernates, Tissue Homogenates
• Sensitivity: 7.81 pg/mL.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: BMP6
• Alias Symbols: Vg, Vgr1, D13Wsu115, D13Wsu115e
• NCBI Gene Id: 12161
• Protein Name: Bone morphogenetic protein 6
• Description of Target: Induces cartilage and bone formation.
• Uniprot ID: P20722
• Protein Accession #: NP_031582
• Nucleotide Accession #: NM_007556