BMP4 ELISA Kit (Mouse) (OKEH02960)

Catalog No: OKEH02960

Size:96 Wells

Mouse BMP4 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology BMP4 ELISA Kit (Mouse) (OKEH02960) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bmp4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Bmp4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Bmp4 captured in well.
• ELISA Kit Range: 15.6 - 1000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 82%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 6.2% (n = 20)
  Mean Inter-assay CV%: <= 9.0% (n = 20)
• ELISA Kit Component:
  • Anti-Bmp4 Microplate: 96 Wells (12 x 8 Well strips)
  • Bmp4 Lyophilized Standard: 2
  • 100X Biotinylated Bmp4 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 7.88 pg/mL
• Specificity: Natural and recombinant Mouse Bone morphogenetic protein 4
• Publications:

  The Wnt Effector TCF7l2 Promotes Oligodendroglial Differentiation by Repressing Autocrine BMP4-Mediated Signaling. J Neurosci. 41, 1650-1664 (2021)

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: BMP4
• Gene Full Name: bone morphogenetic protein 4
• Alias Symbols: Bmp-, Bmp2, Bmp-4, Bmp2b, Bmp2b-, Bmp2b1, Bmp2b-1
• NCBI Gene Id: 12159
• Protein Name: Bone morphogenetic protein 4
• Description of Target: Induces cartilage and bone formation. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.3 Publications
• Uniprot ID: P21275
• Protein Accession #: NP_001303289.1
• Nucleotide Accession #: NM_001316360.1
• Protein Size (# AA): 408
• Molecular Weight: 46 kDa