Catalog No: OKEH02960
Price: $450.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH02960
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology BMP4 ELISA Kit (Mouse) (OKEH02960) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bmp4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Bmp4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Bmp4 captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 82%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.2% (n = 20)
Mean Inter-assay CV%: <= 9.0% (n = 20)
ELISA Kit Component
Anti-Bmp4 Microplate96 Wells (12 x 8 Well strips)
Bmp4 Lyophilized Standard2
100X Biotinylated Bmp4 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity7.88 pg/mL
SpecificityNatural and recombinant Mouse Bone morphogenetic protein 4

The Wnt Effector TCF7l2 Promotes Oligodendroglial Differentiation by Repressing Autocrine BMP4-Mediated Signaling. J Neurosci. 41, 1650-1664 (2021)

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolBmp4
Gene Full Namebone morphogenetic protein 4
Alias SymbolsBmp-;Bmp2;Bmp2b;Bmp2b-;Bmp2b1;Bmp2b-1;Bmp-4;bone morphogenetic protein 2B;bone morphogenetic protein 4.
NCBI Gene Id12159
Protein NameP21275
Description of TargetBone morphogenetic protein 4
Uniprot IDhttps://www.uniprot.org/uniprot/P21275
Protein Accession #NP_001303289.1
Nucleotide Accession #NM_001316360.1
Protein Size (# AA)408
Molecular Weight46 kDa
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